Effect of tropical forage species in silvopastoral arrangements on methane production and in vitro fermentation parameters in a RUSITEC system

Background: Supplementation of grazing cattle with native and naturalized forages using silvopastoral systems has been suggested as an affordable strategy to reduce methane production improve nutrition, diminishing the environmental impact production. Objective: To evaluate effect three tropical forage species in a arrangement on fermentation parameters vitro ruminal simulation system (RUSITEC). Methods: Four diets were evaluated. The control treatment was basal diet colosuana grass (COL; Bothriochloa pertusa), while other consisted 70% COL complemented 30% shrub from either Leucaena leucocephala (CL), Guazuma ulmifolia (CG), or Crescentia cujete (CT). A randomized complete block design repeated measurements over time used. Results: inclusion did not affect pH, organic matter degradation (OMD) volatile fatty acids (VFA). affected structural components. concentration N-NH3 increased CL compared (p<0.05). In general, terms mL/day, mL/g DMi, DMd, mL/gOMd reduced for Conclusions: Based these results, leucocephala, B. pertusa-based improves reduces